ZFIN Image: Wen et al., 2013, Fig. 5

Image

Figure Caption

Fig. 5

Calcium signal onset is delayed at boutons in ω-conotoxin GVIA-treated fish.

(A and C) Sample images of 100 Hz stimulus evoked Fluo-5F fluorescence increases taken at 1 s intervals for 4 ROIs for control (A) and ω-conotoxin GVIA-treated (C) fish. The scale bar corresponds to 20 µm. (B and D) The stimulus-driven fluorescence increases associated with each color-coded ROI in control (B) and ω-conotoxin GVIA-treated (D) fish. The fluorescence was baseline subtracted and the increase was expressed as ΔG/R. Black bars in (B) and (D) indicate the timing of 100 Hz stimulation. Experiments were performed with 0.5 mM EGTA in the intracellular solution. The entire videos for A and C are available as Video 1 and Video 2 respectively. For each video the timing of stimulation is indicated by the dot.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife