ZFIN Image: Lepage et al., 2014, Fig. 3

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Figure Caption

Fig. 3

Dynamin-dependence of fluid-phase endocytosis at the blastoderm margin. ((A)(D)) The effect of dynasore treatment ((A) and (B)) and global DN-Dnm2 ((C) and (D)) expression on the uptake of a fluorescent fluid-phase marker, FITC. All embryos are globally expressing mRFP to show the position of the EVL margin. Arrow in (B) shows the passage of our fluid-phase marker into the deep cell layer, while arrowheads indicate cells which have passed the EVL margin. ((E) and (F)) Quantification of vesicle number in embryos treated with dynasore and those expressing DN-Dnm2. Bars represent the least squares mean vesicle number±standard error. Statistical significance was tested using two-way ANOVAs. Dynasore treatment: F_3,60=43.90, P<0.0001; Day: F_5,60=18.01, P<0.0001. A Tukey HSD test indicated the control differed significantly from dynasore treatment in both control injected and dn-rho RNA injected groups. Global DN-Dnm2: F_1,14=16.63, P=0.0011; Day: F_2,14=5.57, P=0.0166. Yolk-biased DN-Dnm2: F_1,36=27.70, P<0.0001; Day: F_4,36=3.05, P=0.029.

Acknowledgments

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Reprinted from Developmental Biology, 385(1), Lepage, S.E., Tada, M., and Bruce, A.E., Zebrafish Dynamin is required for maintenance of enveloping layer integrity and the progression of epiboly, 52-66, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.