Fig. 2

Fig. 2

Dynamin-dependent fluid-phase endocytosis within the yolk cell is dispensable for epiboly progression. ((A)(D)) Live images of epiboly in a representative uninjected embryo ((A)(A′′)), control injected embryo ((B)(B′′)) and embryos expressing DN-Dnm2 globally ((C)(C′′)) or in a yolk-biased manner ((D)(D′′)). Embryos are shown in lateral view at approximately 30% epiboly ((A)(D)), 60% epiboly ((A2)(D′)) and at bud stage ((A′′)(D′′)). Arrows in ((A′)(D′)) indicate the position of the advancing blastoderm margin. (E) Schematic diagram of global versus yolk-biased injection method. ((F) and (G)) Quantification of the effect of global and yolk-biased DN-Dnm2 expression on epiboly progression. Bars represent the mean percentage of yolk coverage. Error bars indicate standard deviation and statistical significance was determined by t-test, ^*P<0.01.