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Fig. 2
Eph-ephrin and pak2a requirements in craniofacial development. (A-H) Alcama immunohistochemistry (green) at 34hpf labels five pouches (p1-p5) in wild types, as well as neuronal ganglia (*), with varying degrees of pouch defects in mutants and transgenic embryos. Scale bar: 40µm. (I-P) Ventral views of facial cartilages. In wild types, a bilateral set of five CBs (5) form posterior to the ceratohyal (CH). Fusions, reductions and losses of CBs are observed in mutants and transgenics, as well as fusions of CH with CB1 (arrows). (Q-S) Immunohistochemistry at 30hpf shows Efnb2a protein (green) in pouches (arrows) and neural-crest-derived mesenchyme (arrowheads). Efnb2a protein is lost in efnb2a mutants and selectively restored to pouch endoderm by the nkx2.3:Efnb2a transgene. (T) Quantification of pouch and CB defects. Data represent mean±s.e.m. ***P<0.001.