ZFIN Image: Hochgreb-Hägele et al., 2015, Fig. 2

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Figure Caption

Fig. 2

Znf385C loss-of-function results in craniofacial defects and premature ossification of the Meckel’s cartilage. (A) Western blot analysis of Znf385C-Citrine fusion in Gt(znf385c-citrine)^ct39a embryos injected with control and translation blocking (atg) morpholinos. Alpha-Tubulin is used as a load control. (B and C) Lateral views. Bright-field of live znf385c morphant (B) and control (C) at 4 dpf. Asterisk indicates cardiac edema. (D–I) Defect in cartilage patterning and maturation in znf385c morphants. Ventral views. (D and E) Alcian blue staining of znf385c morphant and control-MO injected embryos at 96 hpf. (F–I) Four classes of jaw phenotypes in control and znf385c atg MO-injected embryos at 5 dpf using the Tg(sox10:EGFP) background to reveal morphology and cellularity of the jaws in vivo. (J–M) Skeletal preparation with Alcian blue and Alizarin red staining at 7 dpf. Lateral (J and L) and ventral (K and M) views. Black arrowheads indicate premature ossification in the MC of znf385c morphants at 7 dpf (J and K), where similar ossification pattern is observed only at 9 dpf in control and wild-type embryos (L and M). Meckel’s cartilage, mc; ceratohyals, ch; pharyngeal arches, pa; quadrate, qu.

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Acknowledgments

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Reprinted from Developmental Biology, 400(1), Hochgreb-Hägele, T., Koo, D.E., Bronner, M.E., Znf385C mediates a novel p53-dependent transcriptional switch to control timing of facial bone formation, 23-32, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.