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Fig. 2, S1
Epithelial flow is independent of cell division.
(A) Retinal cell division was inhibited by application of aphidicolin, a well-established DNA polymerase inhibitor. Aphidicolin efficiently inhibited cell proliferation shown by drastically reduced pHH3 positive nuclei (upper panel, average of 6 pHH3 positive nuclei) compared to the control (lower panel, average of 21 pHH3 positive nuclei) (the optic cup is encircled with a dotted white line, 21.5 hpf). (B) We addressed the epithelial flow of aphidicolin-treated wild-type embryos, injected with H2BGFP RNA at the one cell stage; please see Figure 2A as control. The embryo was preincubated with aphidicolin 5 hr prior to the start of imaging (17 hpf, see also Video 2). The application of aphidicolin did not affect the epithelial flow. As a low level side effect of aphidicolin we observed cell death, in line with previous reports, importantly also not affecting the epithelial flow.