ZFIN Image: Cheng et al., 2015, Fig. S2

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Figure Caption

Fig. S2

sim1a morphants show increased cell death and reduced heart rate. (A) sim1a ATG morphant embryos exhibit increased levels of cell death as indicated by acridine orange stains (asterisk) when compared to wild-type controls and embryos injected with the sim1a 5 bp ATG mmMO. (B) Live time course of embryos injected with the sim1a 5 bp ATG mmMO as a control for assessing the specificity of the sim1a translation-targeting MO. Embryo anterior is located to the left in all panels. (C) Graph depicts the mean heartbeats per minute for wild-type (WT) embryos and sim1a ATG morphants at 38, 48, and 72 hpf. Asterisks indicate the degree of significance: p<0.05*, 0.01**, 0.001***. Abbreviations: bp – base pair; hpf – hours post fertilization; mmMO – mismatch morpholino; MO – morpholino; WT – wild-type.

Acknowledgments

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Reprinted from Developmental Biology, 399(1), Cheng, C.N., Wingert, R.A., Nephron proximal tubule patterning and corpuscles of Stannius formation are regulated by the sim1a transcription factor and retinoic acid in zebrafish, 100-16, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.