ZFIN Image: Renz et al., 2015, Fig. 6

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Figure Caption

Fig. 6

Misexpression of KLF2 Depends on Integrin β1

(A) qRT-PCR shows that elevated expression of KLF2 mRNA in ICAP1-, KRIT1-, or CCM2-silenced HUVECs is partly suppressed upon simultaneously interfering with β1 integrin (ITGB1).

(B–D) Morpholino knockdown of zebrafish integrinβ1b completely restores cardiac morphology (marked by Tg(kdrl:GFP)^s843 and actin staining) and cardiac cushion formation in zebrafish ccm2^m201 mutants at 48 hrpf. (B′–D′) Shown are single confocal plane sections with details of the AVC region (white box in B–D) with endocardial cushion cells as marked by actin (asterisks).

(E) Model of CCM/ICAP1-controlled proangiogenic activity of the β1 integrin-Klf2 mechanotransduction pathway. Endo, endocardium; myo, myocardium. Scale bars, 25 µm. Statistical data are means and SEM; ns, not significant;p < 0.001; p < 0.0001; see Supplemental Experimental Procedures for details about statistical data analysis.

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Acknowledgments

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Reprinted from Developmental Cell, 32, Renz, M., Otten, C., Faurobert, E., Rudolph, F., Zhu, Y., Boulday, G., Duchene, J., Mickoleit, M., Dietrich, A.C., Ramspacher, C., Steed, E., Manet-Dupé, S., Benz, A., Hassel, D., Vermot, J., Huisken, J., Tournier-Lasserve, E., Felbor, U., Sure, U., Albiges-Rizo, C., Abdelilah-Seyfried, S., Regulation of β1 Integrin-Klf2-Mediated Angiogenesis by CCM Proteins, 181-190, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell