ZFIN Image: Renz et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Elevated Expression Levels of egfl7 mRNA Are Involved in the ccm2^m201 Mutant Phenotype

(A) Loss of Ccm2 or overexpression of klf2a/b in Tg(hsp70l:klf2a)^md8 or Tg(hsp70l:klf2b)^md9 transgenic embryos causes elevated levels of egfl7 mRNA, as determined by qRT-PCR.

(B) Similarly, EGFL7 mRNA is upregulated in HUVECs upon knockdown of ICAP1, KRIT1, or CCM2 by siRNA treatment.

(C-E′) Cardiac morphology and cardiac cushion formation are rescued by egfl7 MO injection (n = 15/15 genotyped ccm2^m201 mutants completely rescued by egfl7 MO). Shown are hearts marked by endocardial Tg(kdrl:GFP)^s843 and actin staining at 48 hrpf or (C′-E′) details with single confocal plane sections of the AVC region. Endocardial cushion cells are marked by the expression of Alcam (inverted image) (asterisks). Scale bars, 25 µm. Statistical data are means and SEM; p < 0.05; p < 0.01; p < 0.001; see Supplemental Experimental Procedures for details about statistical data analysis.

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Acknowledgments

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Reprinted from Developmental Cell, 32, Renz, M., Otten, C., Faurobert, E., Rudolph, F., Zhu, Y., Boulday, G., Duchene, J., Mickoleit, M., Dietrich, A.C., Ramspacher, C., Steed, E., Manet-Dupé, S., Benz, A., Hassel, D., Vermot, J., Huisken, J., Tournier-Lasserve, E., Felbor, U., Sure, U., Albiges-Rizo, C., Abdelilah-Seyfried, S., Regulation of β1 Integrin-Klf2-Mediated Angiogenesis by CCM Proteins, 181-190, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell