ZFIN Image: Renz et al., 2015, Fig. 3

Image

Figure Caption

Fig. 3

Enhanced VEGFR Signaling Is Involved in the Zebrafish ccm2^m201 Mutant Cardiac Phenotype

(A–C) Rescue of cardiac malformations in ccm2^m201 mutants at 48 hrpf after treatment with the VEGFR inhibitor PTK787 between 15 and 17 hrpf. (A–C) Shown are hearts marked by endocardial Tg(kdrl:GFP)^s843 and actin staining at 48 hrpf or (A′, B′, and C′) details with single confocal plane sections of the AVC region (white box in A, B, and C). Endocardial cushion cells are characterized by their cuboidal shape (asterisks) (n = 5/5 genotyped ccm2^m201 mutants completely rescued by PTK787 treatment).

(D) Treatment of ccm2^m201 mutants with PTK787 between 15 and 17 hrpf significantly reduces the endocardial overproliferation phenotype, and treatment between 24 and 48 hrpf suppresses the overgrowth of the LDA.

(E) The phenotypic rescue of ccm2^m201 mutants by treatment with PTK787 between 15 and 48 hrpf does not affect the elevated expression levels of klf2a and klf2b as detected by qRT-PCR. Endo, endocardium; myo, myocardium. Scale bars, 25 µm. Statistical data are means and SEM; ns, not significant; p < 0.05; p < 0.01; p < 0.001; p < 0.0001; see Supplemental Experimental Procedures for details about statistical data analysis.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 32, Renz, M., Otten, C., Faurobert, E., Rudolph, F., Zhu, Y., Boulday, G., Duchene, J., Mickoleit, M., Dietrich, A.C., Ramspacher, C., Steed, E., Manet-Dupé, S., Benz, A., Hassel, D., Vermot, J., Huisken, J., Tournier-Lasserve, E., Felbor, U., Sure, U., Albiges-Rizo, C., Abdelilah-Seyfried, S., Regulation of β1 Integrin-Klf2-Mediated Angiogenesis by CCM Proteins, 181-190, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell