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Fig. 6
Crosstalk among HB-EGF, Insulin, IGF-1/FGF, and IL-11/Leptin Signaling Components Contributes to MG Proliferation in the Uninjured Retina
(A) RT-PCR shows the effect of intravitreal injection of HB-EGF, Insulin, IGF-1/FGF2, or Leptin/IL-11 on each other’s expression and also the regeneration-associated factor, wnt8b.
(B) BrdU immunofluorescence shows that MO-mediated knockdown of specific HB-EGF, Insulin, and IGF-1 signaling components suppress each other’s ability to stimulate MG proliferation in the uninjured retina. Scale bar represents 50 µm.
(C–E) Quantification of data presented in (B). Error bars represent SD; p < 0.001; n = 4.
(F) Conditional overexpression of dnFgfr1 in hsp70:dnfgfr1-egfp fish inhibits FGF2/IGF-1-dependent MG proliferation, but not Insulin or HB-EGF-dependent MG proliferation, in the uninjured retina. Scale bar represents 150 µm.
(G) Quantification of data presented in (F). Error bars represent SD. p < 0.001; n = 3.
See also Figure S6.