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Fig. 5
HB-EGF and IL-11 Synergize with Each Other to Stimulate MG Proliferation and Activation of the Stat3 and β-Catenin Signaling Components in the Uninjured Retina
(A and B) HB-EGF and IL-11 synergize with each other to stimulate the generation of BrdU+ progenitors and the accumulation of Stat3 (A) and β-catenin (B) signaling components in the uninjured retina of gfap:stat3-gfp transgenic fish. Arrows point to double-labeled cells. Scale bar represents 50 µm. The green immunofluorescence signal in the photoreceptor layer is nonspecific, since it does not show up when using a secondary antibody coupled to a red Fluor (Figure S7).
(C–E) Quantification of BrdU+ (C), Stat3-GFP+ (D), and β-catenin+ (E) progenitors in retinas intravitreally injected with HB-EGF, IL-11, or HB-EGF/IL-11. Error bars represent SD; n = 3.
See also Figure S5.