ZFIN Image: Zhao et al., 2014, Fig. 6

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Figure Caption

Fig. 6

Leptin Synergizes with IL-11 and CNTF to Stimulate MG Reprogramming and Proliferation in the Uninjured Retina

(A) GFP and BrdU immunofluorescence shows that Leptin synergized with IL-11 and CNTF to stimulate GFP expression and MG proliferation in the uninjured retina 1016tuba1a:gfp fish, whereas Leptin, IL-11, or CNTF alone had little effect. Arrows point to MG-derived progenitors in the top three panels.

(B) Quantification of the effects of cytokines on MG proliferation when delivered individually or in combination to the uninjured retina. p < 0.001 (combination versus individual), n = 4 per group.

(C and D) MO-mediated Ascl1a knockdown inhibits the synergistic effects of Leptin/IL-11 or Leptin/CNTF on GFP induction and MG proliferation. p < 0.05, n = 3.

(E and F) Knockdown of Gp130 or Lepr inhibits the synergistic effects of Leptin/IL-11 on proliferation. p < 0.001, n = 4.

Error bars, SD. Scale bars, 50 µm (A, C, and E).

Acknowledgments

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