Fig. 7

Fig. 7

Effect of Model Parameters on Epithelial Morphogenesis

(A–D) Interplay model simulations of <L/R> using one variable parameter while fixing other parameters as in Figure 5C.

(E) Crb and control phenotypes at sphere stage. xCrb-GFP is visible as diffuse GFP signal in the image. Embryos are transgenic for H2B-GFP and mem-mCherry2. Scale bar represents 50 µm.

(F) L/R ratio measurements for embryos in (E). Error bars are SD.

(G) N_S trends in Crb embryos. The wild-type (WT) data is from Figure S2H. At ~2 k cell stage, two Crb+ data points are larger than 2× SD of the WT and two are larger than 1× SD (p = 0.002). Error bars are SD.

(H) Division rule in Crb injected cells. n = 122. In mother cells that satisfy 1 < L/R < 1.3, SS:SD for Crb+ cells is 17:27 (due to oblique divisions) whereas for WT is 11:39. See also Figures S7E and S7G.

(I) Interplay model prediction of Crb+ dynamics. For simplicity, the only changed parameter compared to WT simulation is Th, despite observations that A, γ also changed slightly in Crb+ embryos. WT data is from Figure 1E. n = 400 for Crb+ data from four movies. Error bars are SEM.

(J) Simulation of sea urchin (Wray 1997) and frog (Chalmers et al., 2003) embryo surface morphogenesis using an altered Th value and different initial conditions (A₁,V_C1,N_S1) and compared to fish. See also Data S1, Text 14.

(K) N_S changes of different species as a result of Th differences. The same Th values as (J) were used. See also Figures S7I and S7J and Data S1, Text 14.