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Fig. 2
Optn is expressed ubiquitously in zebrafish embryos.
Whole-mount images of in situ hybridization for optn in zebrafish embryos at 24 hpf (A, B) and 4 dpf (C, D). A and C are lateral mounts with anterior to the left and dorsal up. B and D are transverse sections of the head and eyes with dorsal up. A, B. optn expression is ubiquitous in the 24 hpf embryos (arrow) with some enrichment in the ventral head (arrowheads). C. optn expression at 4 dpf shows staining in the head (arrowhead) and higher enrichment in the otic vesicle (ov), swim bladder (sb) and intestine (i). D. Within the 4 dpf eye, optn expression is enriched in the retinal ganglion cell (arrow) and inner nuclear (arrowhead) layers.