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Fig. 5
Immunocytochemical analyses of Sycp1 and Sycp3 in primary spermatocytes. Localization of Sycp1 (A–E), Sycp3 (F–J), merged Sycp1 and Sycp3 staining (K–O), and merged Sycp1, Sycp3, and nuclear staining with TO-PRO-3 (P–T) are shown at the preleptotene (A,F,K,P), early leptotene (B,G,L,Q), early zygotene (C,H,N,R), zygotene (D,I,M,S), and pachytene (E,J,O,T) stages. Each developmental stage of spermatocytes was identified according to their synaptonemal complex (SC) structures. Bar = 10 µm.
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