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Fig. S6
gpr22 morphants display otolith defects. Left ear with anterior to the left of zebrafish embryos at 2 dpf, injected at one cell stage with (A) water or (B, C) 0.2 mM gpr22 MO. (A) Control embryos have 2 tethered otoliths (white asterisks) at the anterior and posterior poles of the otic vesicle. In contrast, gpr22 morphants display (B) fused otoliths or (C) an increased number of otoliths, which are not correctly positioned. dpf = days post-fertilization, MO = morpholino.
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