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Fig. S1
gpr22 knock down results in a dose-dependent curvature of the tail. (A–C) Lateral views with anterior to the left of zebrafish embryos at 24 hpf, injected at one cell stage with (A) water, (B) 0.2 mM gpr22 MO1 with 0.1 mM p53 MO or (C) without p53 MO. (D) Quantification of the tail phenotypes. (A, B, D) Knock down of gpr22 with either MO1 or MO2, results in a dose-dependent WT, mild, medium or severe downwards curvature of the tail. (B, C) Co-injection with p53 MO rescues the head necrosis caused by MO1. MO = morpholino, hpf = hours post-fertilization, n = number of analyzed embryos, WT = wild type.
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