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Fig. 6
Gpr22 does not act upstream of Foxj1a/Rfx2 in KV ciliogenesis.
(A, B) WISH of (A) foxj1a or (B) rfx2 on embryos at bud stage. Shown are (A) the lateral view with anterior to the left or (B) dorsal view with anterior to the top. gpr22 knock down or overexpression does not alter the DFC expression of foxj1a or rfx2, two master regulators of ciliogenesis. (C) Confirmation by qPCR of the foxj1a, rfx2 and polaris (target gene) RNA levels in the whole embryo, normalized to beta-actin. MO = morpholino, WISH = whole-mount in situ hybridization, DFC = dorsal forerunner cell, KV = Kupffer’s vesicle.
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