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Fig. 11 Knockdown of miR-2190 does not affect proliferation. Adult albino zebrafish were intravitreally injected and electroporated with either standard control or miR-2190 morpholino prior to the start of light damage. A,B: After 51h of constant light, standard control and miR-2190 morphant retinas both display PCNA-positive doublet INL proliferating nuclei (arrows). C,D: After 72h of light, standard control and miR-2190 morphant retinas display clusters of INL nuclei (arrowheads). E,F: Statistically equivalent numbers of INL and ONL PCNA-positive nuclei are present in standard control and miR-2190 morphant retinas. INL, inner nuclear layer; ONL, outer nuclear layer. Scale bar in A = 50 µm and applies to B–D; data represent mean ± s.e.m. P > 0.05 using two-way ANOVA with Tukey′s post-hoc test, n=6.