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Fig. S5 Expression of otx1b and tbx1 in lia (fgf3-/-) and ace (fgf8-/-) mutant embryos. (A, D) Expression of otx1b is normal in lia sibling (n = 65/85 embryos from a heterozygous cross) and ace sibling (n = 64/91) embryos. (B) The expression domain and levels of otx1b are reduced in the lia mutant otic vesicle (n = 20/85). (C) The expression domain of otxb1 is reduced in the much smaller ear of ace mutant (n = 27/91) embryos, but the overall pattern and level are normal. (E, H) Expression of tbx1 is normal in lia sibling (n = 64/87) and ace sibling (n = 59/82) embryos. (F) Expression of tbx1 is expanded anteroventrally in the OV of lia mutant (n = 23/87) embryos, filling in the anteroventral zone that is normally free of tbx1 expression. (G) The pattern of tbx1 expression is almost normal in ace mutant (n = 23/82) embryos, despite the smaller size of the OV. All panels are lateral views with anterior to the left. Scale bar: 50 µm.