ZFIN Image: Casari et al., 2014, Fig. S6

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Figure Caption

Fig. S6 Tg(12xSBE:EGFP)ia16 line is responsive to the overexpression of smad3b, and smad3a together with smad3b: effects of mRNA injection on embryos with a malformed phenotype. Brightfield and fluorescent images of head and tail of 24 hpf embryos injected with smad2, 3a, 3b and 4 mRNAs. The last column shows a zoomed view of the tails. White arrow heads in fluorescent head pictures point out the cardiac mesoderm. Injection of smad3b and smad3a+smad3b mRNAs causes an increase of GFP expression in this area. In the same injected embryos overexpression of GFP is also visible in tail mesoderm (white arrow heads).

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Reprinted from Developmental Biology, 396(1), Casari, A., Schiavone, M., Facchinello, N., Vettori, A., Meyer, D., Tiso, N., Moro, E., Argenton, F., A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development, 81-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.