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Fig. 1 Morpholino-mediated knockdown of Dicer protein expression. Lissamine-tagged Standard Control morpholino (Std Ctl MO) or dicer morpholino were intravitreally injected and electroporated into dark-adapted albino retinas. After 35h constant light, dosal retinas were isolated and the expression of Dicer and Actin were analyzed by immunoblots (A). After incubation with the secondary antibody, the membrane was separated to allow a longer exposure of the Dicer region relative to the Actin region. After adjusting for the difference in the amount of the Actin protein in the two samples, the dicer morphant retinas possessed 77% less Dicer protein relative to the Standard Control morphant retina (B, *P<0.05, n=5).