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Fig. 6
etv7 regulates red blood cells through the cholesterol synthesis pathway. (A) Real-time PCR analysis of lss expression in embryos injected with either 8.2 ng of standard control morpholino (Std) or 8.2 ng of etv7 morpholinos (MT ATG). Error bars represent standard deviation. (B) Inhibition of Lss enzymatic activity phenocopies knockdown of etv7. Wild-type embryos were treated with the Lss inhibitor Ro 48-8.071 (50 nM) and stained with o-dianisidine at 2 dpf. Injection of etv7 morpholinos was performed simultaneously and compared with embryos treated with Ro 48-8.071. Rescue experiments were performed by injecting cholesterol into the yolk of embryos at 1 dpf. o-dianisidine staining was used to visualize the presence or absence of hemoglobinized red blood cells at 2 dpf (Std n=26, Std with cholesterol n=32, MT ATG n=20, MT ATG with cholesterol n=15). Refer to Table 1 for P-values and percent affected in each category.