ZFIN Image: Campbell et al., 2013, Fig. 7

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Figure Caption

Fig. 7 p38 MAPK interacts with Slit1a-Robo2 signaling to restrict arbor and presynaptic growth. (A–F) Maximum intensity projections of 80-hpf RGC arbors in the optic tectum expressing YFP-Rab3. coMO (A), p38mapkMO1 (B), slit1a transMO1 (low dose; C), robo2SBMO1 (low dose; D), p38mapkMO1; slit1a transMO1 (low dose; E), and p38mapkMO1; robo2SBMO1 (low dose; F). (G–L) Quantification of arbor parameters: branch tip number (G), arbor area (H), total arbor length (I), arbor order (J), presynaptic puncta number (K), and puncta density (L). 6–20 arbors were analyzed per condition. Dorsal views are shown, and anterior is up. White asterisks indicate the parent axon. Error bars represent SEMs. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bar, 10 μm.

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Acknowledgments

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