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Fig. S8 Immunolabeling of Pcdh15a in wild-type siblings and pcdh15apsi7 mutants. (A) Sequence of the psi7 allele, which encodes splice site mutation causing a frameshift following exon 7. (B) The predicted product is a short N-terminal fragment of Pcdh15a. (C) Immunolabeling with a monoclonal antibody against Pcdh15a. Actin filaments were labeled with rhodamine–phalloidin. (Right) Note the splaying of stereocilia, which is typical for mutants expressing strong alleles of pcdh15a (1). (Scale bar: 5 μm.)
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Full text @ Proc. Natl. Acad. Sci. USA