ZFIN Image: Choe et al., 2014, Fig. S6

Image

Figure Caption

Fig. S6 Fgf activity in the endoderm of wnt11r mutants
(A and B) Confocal projections show Fgf activity as marked by dusp6:dGFP (green) relative to the her5:mCherryCAAX+ pouch endoderm (red) at 32 hpf. In 8/8 wnt11r mutants, dusp6:GFP expression is grossly unaffected compared to wildtype siblings (n=34). Scale bar represents 40 μM. (C and D) Higher magnification and gain-enhanced sections focused on the posterior pouches seen in (A) and (B) show dusp6:dGFP fluorescence within alternating clusters of endodermal epithelial cells (arrowheads), as well as in clusters of adjacent mesodermal cells (asterisks). While pouch outpocketing is delayed in wnt11r mutants, segmental dusp6:dGFP fluorescence is still detectable within both the mesoderm and endoderm. Scale bar represents 20 μM.

Acknowledgments

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