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Fig. S8 Apoptosis is not responsible for the loss of posterior structures in smp morphants, Related to Figure 1. (A) Measurement of TUNEL-positive cells in mismatch (MM-MO) control embryos and smp morphants (AS-MO). (B) Measurement of Annexin-Vpositive cells in MM-control embryos and smp morphants. (C) Graph showing the effect of p53 morpholino knockdown in MM-control and and smp-morphant embryos. (D) A 10-hour old p53-knockdown larva shows no effect on the expression of the 7xTCF:mCherry reporter. (E) smp-p53 double knockdown shows that loss of p53 does not restore the expression of the Wnt-dependent reporter in embryos lacking smp. (F) A smp morphant at 10 hours post fertilization shows loss of report expression. Scale bars equal 100 μm.