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Fig. S5
Mcc and Scribble are not in a simple genetic pathway. (A) Overexpression of zebrafish scrib mRNA yields minor CE defects—shortened body axis, displaced head, slight ventral flexure—consistent with published reports (Li et al., 2013; Wada et al., 2005). Co-injection of scrib mRNA with either wnt5b, ror2 or mcc morpholinos results in highly penetrant CE phenotypes that strongly resemble those of the individual morphants (Figs. 2A,B and 3D,E; Supp. Figs. 2A and 3A), indicating no rescue by scrib mRNA. Co-injection of vangl2 MO and scrib mRNA, however, yields a strong genetic interaction (enhanced CE phenotype) that has been previously documented (Wada et al., 2005). Note the further diminished anterior development, slight yolk extension and significantly shortened overall body axis length. (B) The weak scrib MO phenotype (Li et al., 2013; Wada et al., 2005) is not rescued by co-injection of mcc mRNA. (C) scrib and mcc MO concentrations that normally do not give rise to CE phenotypes are denoted with an asterisk (MO* and MO1*). 93% and 85% of scrib MO* and mcc MO1* singly injected embryos, respectively, display wild-type morphology. In contrast, co-injection of scrib MO* and mcc MO1* unmasks a strong “synthetic” interaction, as the doubly morphant embryos show greatly diminished anterior development (a hammerhead phenotype) and foreshortened body axis. This result provides strong evidence that Mcc and Scrib act in parallel signaling pathways that converge on the regulation of similar cellular processes. (AC) Morpholino and mRNA concentrations are provided in Supp. Table 3. Phenotypic distributions are indicated as percentages, with scored embryo counts listed in Supp. Table 4.