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Fig. 4

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ZDB-IMAGE-141124-4
Genes
Antibodies
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Figures for Schindler et al., 2014
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Figure Caption

Fig. 4

Overexpression of hand2 causes outflow tract expansion. (A-H) MF20 antibody staining (red) and mef2cb in situ hybridization (green) at 36hpf in (A,B) nontransgenic, (C,D) Tg(hsp70:hand2), (E,F) Tg(hsp70:hand2P) and (G,H) Tg(hsp70:hand2AA) embryos, all of which were heat-shocked at 10hpf. Frontal views; (A,C,E,G) partial reconstructions of confocal z-stacks with arrows indicating outflow tract; (B,D,F,H) single slices with brackets marking outflow tract. Embryos overexpressing hand2 (C,D) or hand2 AA (G,H) exhibit expanded populations of mef2cb-expressing cells, whereas embryos overexpressing hand2 P (E,F) do not.

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