Fig. S12

Fig. S12

Ectopic expression of Dkk1b-mTangerine results in decreased NM number and pLL truncation (A-D) Confocal projections of 2 dpf embryos with ectopic expression Dkk1bmTangerine (red) driven by heat-shock at 25 hpf. (A) Uninjected wild-type embryo show full pLL extension and terminal cluster (tc) formation, while uninjected krm1^nl10 mutants show pLL truncation (B; yellow arrowhead). Dkk1bmTangerine expression results in truncated pLL extension in both wild-type (C; yellow arrowhead) and krm1^nl10 (D; yellow arrowhead) embryos. Scale bars=20 μm. (E) Quantification of NM number at 2 dpf; there is a significant decrease in NM between uninjected control embryos (wild-type and krm1^nl10 mutants) and embryos expressing Dkk1b-mTangerine (n=15, **p<0.001, two-way ANOVA with replication). There was no difference in NM number between wild-type and mutants expressing Dkk1b-mTangerine (n=15; Tukey’s Post-Hoc Test).