Fig. S10

Fig. S10

Cellular proliferation is not rescued by treatment with SU5402 (A-D′) Confocal projections of BrdU incorporation (red) in wild-type and krm1ⁿ¹⁰ embryos treated with DMSO or SU5402 between 24 and 34 hpf. BrdU label is present in the leading pLLP cells in DMSO treated wild-type (A-A′), but not mutant embryos (C-C′). SU5402 treatment resulted in reduced leading region BrdU incorporation in wild-type embryos (B-B′), but incorporation was not altered in SU5402 treated krm1^nl10 mutants (D-D′). Scale bars=20 μm. (E) Quantification of the index of total BrdU incorporation in the pLLP showing no significant change in control versus treated wild-type embryos or control versus treated krm1^nl10 mutants (n=11 embryos per condition, Student′s t-test). (E) Quantification of the index of BrdU incorporation in the leading region of the pLLP shows a significant decrease in SU5402 treated wild-type embryos, but no change in krm1^nl10 mutants (n=11 embryos per condition, Student′s t-test).