|
Fig. S2
The hit compounds increase the number of beta-cells undergoing S-phase. (A) Tg(ins:Kaede) larvae were treated from 3 to 5 dpf with 1% DMSO, 1 μM retinoic acid, 10 μM trazodone, or 10 μM prednisolone in the presence of 2.5 mM EdU. The numbers of Tg(ins:Kaede)+(green) and EdU+ (red) beta-cells were increased in the animals treated with the hit compounds as compared to DMSO-controls. (B) Quantification of the number of Tg(ins:Kaede)+and EdU+ beta-cells. Retinoic acid (n = 18 animals) and prednisolone (n = 16 animals) significantly increased the number of EdU+ beta-cells compared to DMSO controls (n = 15 animals). Trazodone (n = 17 animals) only mildly increased the number of EdU+ beta-cell compared to DMSO controls consistent with its less potent effect on beta-cell proliferation (see Table 1); this effect was not statistically significant (N.S.) (p = 0.46). *p<0.05 and **p<0.01. Error bars represent SEM.