Fig. S2

Wnt signaling in the pharyngeal endoderm is necessary for D-V arch patterning. (A) Dissected, flat mounted alcian blue/alizarin stained cartilage/bone at 5 dpf. Anterior to the left. Transplantation of dntcf3+ endoderm results in D-V patterning defects including reduced Mc, fused Mc-Pq, and loss of Hs. A dotted line indicates the extent of the endoderm transplant, which was lost in dissection. Asterisk indicates joint fusion. (B–C) Lateral views of in situ hybridization for jag1b in a control (B) and a mosaic embryo in which dntcf3+ cells were transplanted into the endoderm in WT hosts. White arrowheads indicate expansion of jag1b expression into the ventral domain of arches 1 and 2. Asterisks indicate transplanted, diaminobenzidine-stained donor cells. Abbreviations: Ch, ceratohyal; Hm, hyomandibular; Hs, hyosymplectic; Mc, Meckel′s; Pq, palatoquadrate.

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