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Fig. S5
E2 up-regulates expression of vtg4 in the liver of 4 dpf DZ zebrafish embryos. Whole-mount ISH was performed with anti-sense vtg4 RNA probes on 4 dpf E2-treated embryos (A) and DMSO-treated embryos (B). ISH of sense vtg4 RNA probes on E2-treated embryos (C) and DMSO-treated embryos were performed as controls. Lateral view; anterior to the left. Arrow (red) indicates expression location of vtg4 in the liver. Scale bars, 200 μm.
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