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Fig. S14
All the erythrocytes present in circulation of 2 dpf zebrafish embryos derive from the primitive wave of hematopoiesis. In order to assess the primitive erythropoiesis, MO injected embryos were stained with O-dianisidine at 2 dpf (arrow). Knockdown of 12 out of 15 candidate genes, namely: akap10, brf1a, brf1b, kalrn1, mfn2, pdia5, psmd13, rcor1, satb1, waspla, wasplb and wdr66, resulted in no observable phenotype. Depletion of brd3a, kalrn2 and kif1b resulted in a severe reduction in the number of primitive erythrocytes. All the embryos are positioned anterior up and dorsal to the back.
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