ZFIN Image: Bielczyk-Maczyńska et al., 2014, Fig. S9

Image

Figure Caption

Fig. S9

MO activities in zebrafish embryos include both sequence-specific RNA binding as well as effects not associated with loss of function of the targeted locus i.e. “off-target” effects. As p53 MO can reduce common off-target effects, the number of thrombocytes was assessed in CHT at 3 dpf in embryos co-injected with p53 MO and gene-specific MOs. Concurrent knock down of p53 with gene-specific MOs did not attenuate the thrombocyte phenotype induced by gene-specific MOs, confirming that the observed decrease in the number of thrombocytes was not induced by off-target effects of MOs. All embryos are oriented with anterior to the left and dorsal to the top.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.