ZFIN Image: Bielczyk-Maczyńska et al., 2014, Fig. S7

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Figure Caption

Fig. S7

To verify that MOs used in this study exerted a specific effect on hematopoiesis we designed a second non-overlapping morpholino (MO2) for all “phenotypic” candidate genes. Splice modifications caused by gene-specific MOs were assayed by RT-PCR, using gene-specific primers, and were confirmed for all but brf1a, kif1b and wasplb MO2. These three MO2 were therefore excluded from further analysis. Injection of the remaining 12 MO2 resulted in the phenotype that was comparable to the one observed with the first MO. Representative fluorescent images of the CHT are shown. All embryos are oriented with anterior to the left and dorsal to the top.

Acknowledgments

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