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Fig. S3
In order to determine the optimal dose of the MOs to be injected, a dose-response experiment was performed. For each gene the concentration of MO (shown in lower right corner of each image, in ng) was selected that elicited a specific phenotype without an overt non-specific effect. Knock-down of grtp1a resulted in early embryonic lethality even when injected with 0.8 ng of MO. Thus, grtp1a was excluded from further analysis. The optimal MO dose for each gene as determined in this experiment was used in all subsequent experiments.
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