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Fig. 31 Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GTCAGTGGACGTGTGGTCAG. Reverse: AATTAACCCTCACTAAAGGGAAGCAAAAGGTGACACATCCA. PCR product size: 811 bp. No expression in the hindbrain. No expression in the spinal cord.
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