ZFIN Image: Sasaki et al., 2014, Fig. S4

Image

Figure Caption

Fig. S4

Effect of UV irradiation on spns1-mutant zebrafish. (A) Acrdine orange (green) and Lysotracker (red) intensities, as well as gross morphology, in wild-type (wt) and spns1-mutant animals treated with UV. The UV (18 mj/cm²) treatment was done at 36 hpf, and phenotypes were observed at 48 hpf. Scale bar, 250 μm. Quantification of data presented in A (n = 9) is shown in the right graphs; the number (n) of animals is for each genotype with or without UV treatment. (B) Cellular characteristics in the animals shown in (A) were observed by using confocal microscopy at high magnification (×600). Scale bar, 10 µm. Quantification of data presented in B (n = 6) is shown in the right graphs; the number (n) of animals is for each genotype with or without UV treatment. Three independent areas (periderm or basal epidermal cells above the eye) were selected from individual animals. Error bars represent the mean ± S.D., *p<0.005; ns, not significant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.