Fig. S9

Fig. S9

Impact of UV-induced apoptosis and autophagy on Spns1 deficiency in the presence or absence of p53. (A) UV-induced apoptosis can be detectable in either spns1^+/+ or spns1^hi891/hi891 animals in similar manners only under the normal p53 condition. The UV (18 mj/cm²) treatment was done at 60 hpf, followed by the phenotype observations in periderm or basal epidermal cells in the caudal eye at 72 hpf. Scale bar in image in top row, 250 µm. Scale bar in image in lower rows, 10 μm. (B) UV-induced autophagy enhances autolysosomal formation in spns1^hi891/hi891 animals in the presence of p53. The UV (18 mj/cm²) treatment was done at 69 hpf, followed by the phenotype observations in periderm or basal epidermal cells in the caudal fin at 72 hpf. Scale bar, 10 µm. (C) Quantification of the EGFP-LC3 and LysoTracker fluorescence intensities shown in (B). Quantification of data presented in panel B (n = 6) is shown in the right graph; the number (n) of animals is for each genotype with MO. Three independent areas (periderm or basal epidermal cells in the caudal fin) were selected from individual animals. Error bars represent the mean ± S.D., *p<0.005; ns, not significant.