ZFIN Image: Carr et al., 2014, Fig. 6

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Figure Caption

Fig. 6

Differential interaction of ZIPK homologs with zebrafish PAR-4. HeLa cells were transfected with rat GFP-ZIPK and flag zebrafish PAR-4 (A, B). The cells were fixed and stained with DAPI and Alexa 568-phalloidin. Black and white images show phalloidin staining, while color images are a merge of DAPI (blue), GFP (green), and phalloidin (red).White bar indicates a 20 μm scale; (C) HEK293T cells were co-transfected with GFP-tagged zebrafish PAR-4 along with flag-tagged ZIPK from human, rat and zebrafish. Protein complexes were immunoprecipitated with anti-FLAG antibodies. The IPs were immunoblotted with anti-GFP antibodies and anti-FLAG antibodies. HC indicates the IgG heavy chain which is the same size as zebrafish and mouse ZIPK, while human is slightly heavier. Each experiment was repeated a minimum of three times.

Acknowledgments

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