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Fig. 1
(A) Non-CT and CT constructs were designed using the Tol2 transgenesis system with the β-actin promoter driving sense transcription and an inverted CMV promoter driving antisense transcription of the mCherry open reading frame (see Supplement for maps and sequence information). All constructs harbored heart-specific GFP (cmlc2-GFP) which enabled identification of transgenic embryos. (B) Widespread expression of mCherry was observed in non-CT embryos (386 out of 386 transgenic embryos), whereas near complete loss of mCherry was observed in CT-mCherry embryos at 2dpf (370 out of 402 transgenic embryos).
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