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Fig. 5 RP loss results in a decrease of IRS1 and phosphorylated AKT substrates. (A) Western blot analysis of IRS1 in GFP-LC3 HEK cells transfected with siScr or siRPS19. (B) Western blot analysis of phosphorylated AKT substrates in GFP-LC3 HEK cells transfected with siScr or siRPS19 and either untreated or stimulated with 350 nM insulin for 6 hours. (C) Densitometer analysis of the total expression level of phosphorylated AKT substrates in (B). (D) Western blot analysis of phosphorylated AKT substrates in 2 dpf zebrafish embryos.
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