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Fig. S1
Two different Stab2 morpholinos result in similar phenotypes. (A–D) In situ hybridization analysis shows kdrl RNA expression at 24 hpf in wild type uninjected (A), standard control morpholino injected (B) and embryos injected with two different Stab2 translation blocking morpholinos (C and D). Kdrl expression was unaffected by control morpholino injection. Stab2 morphants display a lack of ISVs. Embryos were microinjected with 3.75 ng of a standard control morpholino, 7.5 ng of Stab2 MO1, or 2.5 ng of Stab2 MO2. Arrows indicate ISVs (A and B) or missing ISVs (C and D).
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