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Fig. S1
yap1 morphants could be partially rescued with yap1 mRNA.
(A) wild type controls and (C) mismatched MO, (B) Translational (5′UTR MO), and (D) splice-site (E1I1 MO) morphants, co-injected with p53 MO, have similar phenotype; curved up trunk, cardiac edema, reduced head and eye size (E) Overexpression of Yap1 caused no obvious phenotype. (F) Partial rescue was achieved by co-injection of E1I1 MO with 150pg of yap1 mRNA. (G) Band-shift of yap1 mRNA transcript (arrow) is observed in RT-PCR of E1I1 morphants (E1I1) as compared to un-injected control (UC) and mismatch control (MM).
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