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Fig. 1
Benzidine staining of Rpl11-deficient zebrafish embryos and the effectiveness of translational inhibition by Rpl11 MO. (A, B, C, D), O-staining shows markedly reduced numbers of hemoglobin-stained blood cells in Rpl11-knockdown embryos; (E, F), The Rpl11–egfp construct was assembled by inserting a partial sequence of Rpl11 cDNA (containing 60 bps from the 52 UTR) and the N-terminus of egfp into modified pEGFP-N1 (the ATG codon of EGFP was removed). The sequence of Rpl11 MO1 compliments 1–24 bp of Rpl11 cDNA. Embryos co-injected with 25 ng Rpl11–EGFP DNA and 0.5 ng control MO expressed EGFP, and Rpl11–EGFP expression was inhibited by co-injection with 0.5 ng Rpl11 MO; B, D, E, and F are the lateral view; A and C are the ventral view.