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Fig. S7
Quantification of apoptosis in WT and MZspg embryos at bud stage. Detection of apoptotic cells by TUNEL staining (A) and subsequent computational image analysis (B). The images show a lateral maximum intensity projection of a z-stack taken from a single embryo. (B) The same z-stack after automatic object recognition using Volocity software (Perkin-Elmer), where most of the apoptotic cells are marked in red (B).
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