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Fig. 3
Def haploinsufficiency results in a failure in the remodelling of the wound epidermis to liver tissue.
(A) Ventral view of the gross morphology of an amputated adult wild-type and def+/-liver 7 days after PH. The dotted white line indicates the amputation site. 1: ventral lobe, 2: left dorsal lobe, 3: right dorsal lobe. (B–D) Representative images of co-immunostaining of PCNA and Bhmt to compare the process of epithelialisation of the amputation site 2 days after PH (B), wound epidermis formation 3 days after PH (C), and wound epidermis remodelling 5.5 days after PH (D) between the wild-type and the def+/- mutant. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). In each case or at each time-point, more than 10 sections from at least three wild-type or def+/- mutant fish were examined. bc, blood clot; lv, liver tissue; we, wound epidermis. Scale bar: 5 mm (A) and 75 μm (B–D).